MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7.

Abstract

Our previous high-throughput sequencing indicated that rno-miR-1298 was down-regulated in ischemia-reperfusion model of rat. However, little is known about the function and molecular mechanism of rno-miR-1298 in rat tumor cell. In this study, rno-miR-1298 was detected to be significantly down-regulated in rat tumor C6 cells. Moreover, overexpression of rno-miR-1298 obviously inhibited the proliferation and induced apoptosis in C6 cells. SET domain containing 7 (SETD 7) was identified to be a target of rno-miR-1298 using bioinformatics and luciferase reporter assays. Overexpression of rno-miR-1298 markedly reduced the expression of SETD 7 at protein level. Knockdown of SETD 7 also suppressed proliferation and promoted apoptosis in C6 cells. It was indicated that rno-miR-1298 affected cell proliferation and apoptosis of rat tumor cells by targeting SETD 7. Thus, the newly identified miR-1298/SETD 7 expands the elaboration of the mechanisms of the development and progression of tumors and may provide therapeutic target for tumors of nervous system.

• Authors: Chun-Mei Wang, Bao-Hua Cheng, Qing-Jie Xue, Jing Chen, Bo Bai
• Journal: International journal of immunopathology and pharmacology (Int J Immunopathol Pharmacol) Vol. 30 Issue 3 Pg. 264-271 (Sep 2017) ISSN: 2058-7384 [Electronic] England
• PMID: 28762861 (Publication Type: Journal Article)

Chemical References

• MIRN1298 microRNA, rat
• MicroRNAs
• Histone-Lysine N-Methyltransferase

Topics

• Animals
• Apoptosis
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Histone-Lysine N-Methyltransferase (genetics, metabolism)
• MicroRNAs (genetics)
• Rats