Adverse effects of chronic exposure to nonylphenol on non-alcoholic fatty liver disease in male rats.

Abstract

Endocrine-disrupting chemical (EDC) has been thought to play a role in non-alcoholic fatty liver disease (NAFLD). However, the toxic effects of Nonylphenol (NP), an EDC, on non-alcoholic fatty liver disease have never been elaborated. This study aimed to investigate whether exposure to NP could induce NAFDL, a promoting effect of high-sucrose-high-fat diet (HSHFD) on the adverse effects caused by NP was evaluated. Fourth eight male rats were assigned to four groups and each group was treated with a specific testing sample: normal-diet (ND) control group (C-ND); normal diet plus NP (180mg/kg/day) group (NP-ND); high-sucrose-high-fat-diet control group (C-HSHFD); HSHFD plus NP (180mg/kg/day) group (NP-HSHFD). At the age of 80 day, sonogram presents diffusely increased hepatic echogenicity in the NP-HSHFD group. The oblique diameter of liver in the NP-HSHFD group was significantly bigger than that in both the C-ND and NP-ND groups. At the age of 90 day, exposure to NP-HSHFD and NP-ND caused a significant increase in NP concentration in liver as compared to the C-ND group. The rats in the groups treated with NP+ND, HSHFD and NP+HSHFD produced significant increases in the body weight, fat weight and FMI, respectively, when compared to the C-ND group. The liver weight and hepatosomatic indexes (HIS) of rats in the NP-HSHFD group are higher than those in the C-HSHFD group. Exposure to NP-HSHFD induced the increases in plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL) as compared to the C-ND group. Morphological examination of liver tissue from rats exposed to NP+HSHFD shown steatosis with marked accumulation of lipid droplets, hepatocellular ballooning degeneration and inflammatory cell infiltration. Chronic exposure to NP might induce NAFLD in male rats. The high-sucrose-high-fat diet accelerates and exacerbates the development of NAFLD caused by NP exposure.

• Authors: Jie Yu, Xuesong Yang, Ya Luo, Xuefeng Yang, Mengxue Yang, Jin Yang, Jie Zhou, Feng Gao, Liting He, Jie Xu
• Journal: PloS one (PLoS One) Vol. 12 Issue 7 Pg. e0180218 ( 2017) ISSN: 1932-6203 [Electronic] United States
• PMID: 28686624 (Publication Type: Journal Article)

Chemical References

• Phenols
• Triglycerides
• nonylphenol
• Cholesterol
• Aspartate Aminotransferases
• Alanine Transaminase

Topics

• Alanine Transaminase (blood)
• Animals
• Aspartate Aminotransferases (blood)
• Cholesterol (blood)
• Diet, High-Fat
• Humans
• Lipid Droplets (drug effects)
• Liver (diagnostic imaging, drug effects, metabolism, pathology)
• Male
• Non-alcoholic Fatty Liver Disease (blood, chemically induced, diagnostic imaging, pathology)
• Phenols (blood, toxicity)
• Rats
• Triglycerides (blood)