Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice.

Abstract

BACKGROUND/AIM:

Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions.

MATERIALS AND METHODS:

Female transgenic (HPV16+/-) and wild-type (HPV16-/-) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR.

RESULTS:

HPV16+/- mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16+/- animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice.

CONCLUSION:

In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.

• Authors: Rui M Gil DA Costa, Rita Araújo, Joana M O Santos, Mara Fernandes, Tiago Neto, Hugo Sousa, Joana Ribeiro, Margarida M S M Bastos, Paula A Oliveira, Diogo Carmo, Fátima Casaca, Sandra Silva, Carlos Lopes, Rui Medeiros
• Journal: Anticancer research (Anticancer Res) Vol. 37 Issue 6 Pg. 2913-2918 (06 2017) ISSN: 1791-7530 [Electronic] Greece
• PMID: 28551628 (Publication Type: Journal Article)
• Copyright: Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Cyclooxygenase 2 Inhibitors
• MicroRNAs
• Mirn146 microRNA, mouse
• Mirn150 microRNA, mouse
• Celecoxib

Topics

• Animals
• Celecoxib (pharmacology)
• Cyclooxygenase 2 Inhibitors (pharmacology)
• Female
• Human papillomavirus 16 (genetics)
• Mice, Transgenic
• MicroRNAs (metabolism)
• Precancerous Conditions (genetics, virology)
• Skin (metabolism, pathology)