Abstract |
Extracellular matrix proteins and their proteolytic products have been shown to modulate cell motility. We have found that certain tumor cells display a chemotactic response to degradation products of the matrix protein elastin, and to an elastin-derived peptide, VGVAPG. The hexapeptide VGVAPG is a particularly potent chemotaxin for lung-colonizing Lewis lung carcinoma cells (line M27), with 5 nM VGVAPG eliciting maximal chemotactic response when assayed in 48-microwell chemotaxis chambers. Binding of the elastin-derived peptide to M27 cells was studied using a tyrosinated analog (Y- VGVAPG) to allow iodination. Scatchard analysis of [125I]Y- VGVAPG binding to viable M27 tumor cells at both 37 and 4 degrees C indicates the presence of a single class of high affinity binding sites. The dissociation constant obtained from these studies (2.7 X 10(-9) M) is equivalent to the concentration of VGVAPG required for chemotactic activity. The receptor molecule was identified as an Mr 59,000 species by covalent cross-linking of the radiolabeled ligand to the M27 tumor cell surface and subsequent analysis of the cross-linked material by electrophoresis and size-exclusion high performance liquid chromatography. These results suggest that M27 tumor cell chemotaxis to VGVAPG is initiated by high affinity binding of the peptide to a distinct cell surface receptor.
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Authors | C H Blood, J Sasse, P Brodt, B R Zetter |
Journal | The Journal of cell biology
(J Cell Biol)
Vol. 107
Issue 5
Pg. 1987-93
(Nov 1988)
ISSN: 0021-9525 [Print] United States |
PMID | 2846590
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Chemotactic Factors
- Cross-Linking Reagents
- Neoplasm Proteins
- Oligopeptides
- Receptors, Cell Surface
- Elastin
- valyl-glycyl-valyl-alanyl-prolyl-glycine
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Topics |
- Animals
- Chemotactic Factors
(metabolism)
- Cross-Linking Reagents
- Elastin
(metabolism)
- Lung Neoplasms
(metabolism)
- Molecular Weight
- Neoplasm Proteins
(metabolism)
- Oligopeptides
(metabolism)
- Receptors, Cell Surface
(metabolism)
- Tumor Cells, Cultured
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