Abstract | OBJECTIVE: To investigate the pharmacokinetics of buprenorphine and its main active metabolite, norbuprenorphine, after administration of an intravenous loading dose followed by constant rate infusion (CRI) in dogs. STUDY DESIGN: Prospective, clinical study. ANIMALS: A total of seven healthy dogs undergoing elective ovariectomy. METHODS:
Buprenorphine was administered as a loading dose (intravenous bolus of 15 μg kg-1) followed by CRI (2.5 μg kg-1 hour-1 for 6 hours). Moreover, intraoperative analgesia was supplemented by an intramuscular carprofen (4 mg kg-1) injection, administered prior to surgery, and by lidocaine, administrated through subcutaneous infiltration and through a splash on the ovarian vascular pedicle during surgery. Pain and sedation were scored for all animals throughout the 24-hour study period and rescue analgesia was administered when a visual analogue scale score was > 40 mm. Blood samples were collected from a jugular catheter at regular intervals, and plasma concentrations of buprenorphine and norbuprenorphine were determined by a validated liquid chromatography-tandem mass spectrometry method. RESULTS:
Buprenorphine showed a two-compartment kinetic profile. Maximum concentration was 23.92 ± 8.64 ng mL-1 at 1 minute (maximum time); elimination half-life was 41.87 ± 17.35 minutes; area under the curve was 486.68 ± 125.66 minutes ng-1 mL-1; clearance was 33.61 ± 13.01 mL minute-1 kg-1, and volume of distribution at steady state was 1.77 ± 0.50 L kg-1. In no case was rescue analgesia required. Norbuprenorphine resulted below the lower limit of quantification in almost all samples. CONCLUSIONS AND CLINICAL RELEVANCE: The results suggest that a buprenorphine CRI can be a useful tool for providing analgesia in postoperative patients, considering its minor side effects and the advantages of a CRI compared to frequent boluses. The negligible contribution of norbuprenorphine to the therapeutic effect was confirmed.
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Authors | Andrea Barbarossa, Julie Rambaldi, Massimo Giunti, Anna Zaghini, Marco Cunto, Daniele Zambelli, Simond Valgimigli, Francesco Santoro, Noemi Romagnoli |
Journal | Veterinary anaesthesia and analgesia
(Vet Anaesth Analg)
Vol. 44
Issue 3
Pg. 435-443
(May 2017)
ISSN: 1467-2995 [Electronic] United States |
PMID | 28416162
(Publication Type: Clinical Study, Journal Article)
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Copyright | Copyright © 2017 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- Analgesics, Opioid
- Carbazoles
- Buprenorphine
- carprofen
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Topics |
- Analgesia
(veterinary)
- Analgesics, Opioid
(administration & dosage, pharmacokinetics)
- Animals
- Buprenorphine
(administration & dosage, pharmacokinetics)
- Carbazoles
(administration & dosage)
- Dogs
- Female
- Ovariectomy
(veterinary)
- Pain Measurement
(veterinary)
- Postoperative Period
- Prospective Studies
- Time Factors
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