Sonophore labeled RGD: a targeted contrast agent for optoacoustic imaging.

Abstract

Optoacoustic imaging is a rapidly expanding field for the diagnosis, characterization, and treatment evaluation of cancer. However, the availability of tumor specific exogenous contrast agents is still limited. Here, we report on a small targeted contrast agent for optoacoustic imaging using a black hole quencher® (BHQ) dye. The sonophore BHQ-1 exhibited strong, concentration-dependent, optoacoustic signals in phantoms, demonstrating its ideal suitability for optoacoustic imaging. After labeling BHQ-1 with cyclic RGD-peptide, BHQ-1-cRGD specifically bound to αvβ3-integrin expressing glioblastoma cell spheroids in vitro. The excellent optoacoustic properties of BHQ-1-cRGD could furthermore be proven in vivo. Together with this emerging imaging modality, our sonophore labeled small peptide probe offers new possibilities for non-invasive detection of molecular structures with high resolution in vivo and furthers the specificity of optoacoustic imaging. Ultimately, the discovery of tailor-made sonophores might offer new avenues for various molecular optoacoustic imaging applications, similar to what we see with fluorescence imaging.

• Authors: Katja Haedicke, Christian Brand, Murad Omar, Vasilis Ntziachristos, Thomas Reiner, Jan Grimm
• Journal: Photoacoustics (Photoacoustics) Vol. 6 Pg. 1-8 (Jun 2017) ISSN: 2213-5979 [Print] Germany
• PMID: 28393018 (Publication Type: Journal Article)