Contact sensitivity induced in mice by methylene bisphenyl diisocyanate.

Abstract

An experimental model of contact sensitivity has been developed in C57BL/6 mice using methylene bisphenyl diisocyanate (MDI), a raw material of polyurethane resins. Sensitization through a single epidermal application of 1% MDI solution in ethyl acetate to the backs of the mice resulted in marked ear swelling. The time course of the swelling was characteristic of delayed-type hypersensitivity and the increment of the ear thickness was compatible with that induced by toluene diisocyanate (TDI). Passive transfer of the MDI-induced contact sensitivity was successfully achieved using lymphocytes from the lymph nodes of MDI-sensitized syngeneic mice, and the effector cells were found to be T cells. Cross reaction between MDI and TDI has shown that MDI is not only a potent contact sensitizer but also can form a contact sensitizer group together with TDI.

• Authors: K Tanaka, A Takeoka, F Nishimura, S Hanada
• Journal: Contact dermatitis (Contact Dermatitis) Vol. 17 Issue 4 Pg. 199-204 (Oct 1987) ISSN: 0105-1873 [Print] England
• PMID: 2827956 (Publication Type: Journal Article)

Chemical References

• Cyanates
• Isocyanates
• Toluene 2,4-Diisocyanate
• 4,4'-diphenylmethane diisocyanate

Topics

• Administration, Cutaneous
• Animals
• Cross Reactions (drug effects)
• Cyanates (pharmacology, toxicity)
• Dermatitis, Contact (etiology, immunology)
• Disease Models, Animal
• Hypersensitivity, Delayed (chemically induced, immunology)
• Immunization, Passive
• Isocyanates
• Male
• Mice
• Mice, Inbred C57BL
• T-Lymphocytes (immunology)
• Toluene 2,4-Diisocyanate (pharmacology)