Cortical dysplasia accounts for at least 14% of
epilepsy cases, and is mostly seen in children. However, the understanding of molecular mechanisms and pathogenesis underlying
cortical dysplasia is limited. The aim of this cross-sectional study is to identify potential key molecules in the mechanisms of
cortical dysplasia by screening the
proteins expressed in brain tissues of childhood
cortical dysplasia patients with
epilepsy using isobaric tags for relative and absolute quantitation-based tandem mass spectrometry compared to controls, and several differentially expressed
proteins that are not reported to be associated with
cortical dysplasia previously were selected for validation using real-time polymerase chain reaction, immunoblotting and immunohistochemistry. 153 out of 3340
proteins were identified differentially expressed between childhood
cortical dysplasia patients and controls. And FSCN1, CRMP1, NDRG1, DPYSL5,
MAP4, and FABP3 were selected for validation and identified to be increased in childhood
cortical dysplasia patients, while PRDX6 and PSAP were identified decreased. This is the first report on differentially expressed
proteins in childhood
cortical dysplasia. We identified differential expression of FSCN1, CRMP1, NDRG1, DPYSL5,
MAP4, FABP3, PRDX6 and PSAP in childhood
cortical dysplasia patients, these
proteins are involved in various processes and have various function. These results may provide new directions or targets for the research of childhood
cortical dysplasia, and may be helpful in revealing molecular mechanisms and pathogenesis and/or pathophysiology of childhood
cortical dysplasia if further investigated.