Abstract | PURPOSE: A rat model was developed to enable direct administration of hyperpolarized 13 C-labeled molecules into a tumor-supplying artery for magnetic resonance spectroscopy (MRS) studies of tumor metabolism. METHODS: Rat P22 sarcomas were implanted into the right inguinal fat pad of BDIX rats such that the developing tumors received their principle blood supply directly from the right superior epigastric artery. Hyperpolarized 13 C-molecules were either infused directly to the tumor through the epigastric artery or systemically through the contralateral femoral vein. Spectroscopic data were obtained on a 7 Tesla preclinical scanner. RESULTS: CONCLUSIONS: The model maximizes tumor substrate/ drug delivery and minimizes T1 relaxation signal losses in addition to systemic toxicity. Therefore, it permits metabolic studies of hyperpolarized substrates with relatively short T1 and opens up the possibility for preclinical studies of hyperpolarized drug molecules. Magn Reson Med 78:2116-2126, 2017. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Authors | Steven Reynolds, Stephen Metcalf, Edward J Cochrane, Rebecca C Collins, Simon Jones, Martyn N J Paley, Gillian M Tozer |
Journal | Magnetic resonance in medicine
(Magn Reson Med)
Vol. 78
Issue 6
Pg. 2116-2126
(Dec 2017)
ISSN: 1522-2594 [Electronic] United States |
PMID | 28191664
(Publication Type: Journal Article)
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Copyright | © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. |
Chemical References |
- Carbon Isotopes
- Stilbenes
- combretastatin A-1
- Pyruvic Acid
- Gadolinium
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Topics |
- Animals
- Arteries
(diagnostic imaging)
- Carbon Isotopes
(chemistry)
- Drug Delivery Systems
- Epigastric Arteries
(diagnostic imaging)
- Female
- Femoral Vein
(diagnostic imaging)
- Gadolinium
(chemistry)
- Magnetic Resonance Spectroscopy
- Male
- Neoplasm Metastasis
- Neoplasms
(diagnostic imaging, metabolism)
- Optical Imaging
- Perfusion
- Phosphorylation
- Pyruvic Acid
(chemistry)
- Rats
- Spectrophotometry
- Stilbenes
(chemistry)
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