Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40).

Abstract

Development of agents for theranostics implies combining the targeting module, the effector module, and the detection module within the same complex or recombinant protein. We have constructed, isolated, and characterized the 4D5scFv-mCherry-PE(40) protein, which exhibits fluorescent properties and specifically binds to cancer cells expressing the HER2 receptor and reduces their viability. The ability of the obtained targeted antitumor agent 4D5scFv-mCherry-PE(40) to selectively stain the HER2-positive cells and its highly selective cytotoxicity against these cells make the obtained targeted recombinant protein 4D5scFv-mCherry-PE(40) a promising theranostic agent for the diagnostics and therapy of HER2-positive human tumors.

• Authors: O N Shilova, E A Souslova, A M Pilunov, S M Deyev, R V Petrov
• Journal: Doklady. Biochemistry and biophysics (Dokl Biochem Biophys) Vol. 471 Issue 1 Pg. 450-453 (Nov 2016) ISSN: 1608-3091 [Electronic] United States
• PMID: 28058684 (Publication Type: Journal Article)

Chemical References

• 4D5scFv-mCherry-PE(40) protein
• Antineoplastic Agents
• Immunotoxins
• Luminescent Proteins
• Recombinant Proteins
• Single-Chain Antibodies
• ERBB2 protein, human
• Receptor, ErbB-2

Topics

• Animals
• Antineoplastic Agents (chemical synthesis, isolation & purification, pharmacology, toxicity)
• CHO Cells
• Cell Line, Tumor
• Cell Survival (drug effects)
• Chromatography, Affinity
• Cricetulus
• Escherichia coli
• Fluorescence
• Genetic Vectors
• Humans
• Immunotoxins (isolation & purification, pharmacology, toxicity)
• Luminescent Proteins (chemical synthesis, isolation & purification, pharmacology, toxicity)
• Microscopy, Fluorescence
• Receptor, ErbB-2 (metabolism)
• Recombinant Proteins (chemical synthesis, isolation & purification, pharmacology, toxicity)
• Single-Chain Antibodies (isolation & purification, pharmacology, toxicity)