The presence of a 33-40 KDa gastrin binding protein on human and mouse colon cancer.

Abstract

Human and mouse colon cancers have specific binding sites for gastrin and demonstrate a trophic response to gastrin. In the present study we used radiolabeled gastrin (2-17), to determine the molecular weight of gastrin binding proteins (receptors) on mouse and human colon cancers, by cross-linking methods. Crude membrane aliquots prepared from the tumors were radiolabeled with [125I]gastrin (2-17) +/- 1000 fold excess of unlabeled gastrin and cross-linked with 1 mM disuccinimidyl suberate. The cross-linked radiolabeled binding protein complexes were solubilized and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. The autoradiographs of the gels demonstrated the presence of a predominant band of approximately 33-40 KDa gastrin binding protein, that was specific for gastrin analogs. Our present findings thus indicate that specific gastrin binding proteins/gastrin receptors on colon cancers are primarily present as one band with a molecular mass of approximately 33-40 KDa and are specific for gastrin-like peptides.

• Authors: L Chicone, S Narayan, C M Townsend Jr, P Singh
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 164 Issue 1 Pg. 512-9 (Oct 16 1989) ISSN: 0006-291X [Print] United States
• PMID: 2803316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Carrier Proteins
• Cross-Linking Reagents
• Gastrins

Topics

• Animals
• Autoradiography
• Carrier Proteins (metabolism)
• Colonic Neoplasms (metabolism)
• Cross-Linking Reagents
• Gastrins (metabolism)
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• Molecular Weight