Increasing circulating exosomes-carrying TRPC5 predicts chemoresistance in metastatic breast cancer patients.

Abstract

Chemoresistance, the major obstacle in breast cancer chemotherapy, results in unnecessary chemotherapy and wasting of medical resources. No feasible method has been available to predict chemoresistance before chemotherapy. In our previous study, elevated expression of transient receptor potential channel TRPC5 was found to be an essential element for chemoresistance in breast cancer cells, and it was determined that it could be transferred to chemosensitive breast cancer cells through releasing extracellular vesicles (EV) containing TRPC5 from chemoresistant cells, resulting in acquired chemoresistance. Exosomes, a type of EV, are secreted membrane-enclosed vesicles of 50-150-nm diameter. In this study we found that circulating exosomes in peripheral blood from breast cancer patients carried TRPC5. In the present study, circulating exosome-carrying TRPC5 (cirExo-TRPC5) level was significantly correlated with TRPC5 expression level in breast cancer tissues and tumor response to chemotherapy. Furthermore, increased cirExo-TRPC5 level after chemotherapy preceded progressive disease (PD) based on imaging examination and strongly predicted acquired chemoresistance. Taken together, our study demonstrated that cirExo-TRPC5 might act as a noninvasive chemoresistance marker and might serve as an adjuvant to the current imaging examination-based chemoresistance.

• Authors: Teng Wang, Kuan Ning, Ting-Xun Lu, Xu Sun, Linfang Jin, Xiaowei Qi, Jian Jin, Dong Hua
• Journal: Cancer science (Cancer Sci) Vol. 108 Issue 3 Pg. 448-454 (Mar 2017) ISSN: 1349-7006 [Electronic] England
• PMID: 28032400 (Publication Type: Journal Article)
• Copyright: © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Chemical References

• Antineoplastic Agents
• Biomarkers, Tumor
• TRPC Cation Channels
• TRPC5 protein, human

Topics

• Adult
• Aged
• Aged, 80 and over
• Antineoplastic Agents (therapeutic use)
• Biomarkers, Tumor (blood)
• Breast Neoplasms (drug therapy, pathology)
• Disease-Free Survival
• Drug Resistance, Neoplasm (physiology)
• Exosomes (metabolism)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Middle Aged
• TRPC Cation Channels (metabolism)