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Specific targeting delivery to MUC1 overexpressing tumors by albumin-chitosan nanoparticles conjugated to DNA aptamer.

Abstract
Chitosan-coated human serum albumin nanoparticles were functionalized by MUC1 aptamer to obtain a selective drug carrier toward cancers overexpressing MUC1. The negative charges of albumin nanoparticles were shifted to positive charges by surface modification with chitosan, and MUC1 was conjugated through an acrylate spacer. The cytotoxicity of targeted nanoparticles was significantly more than non-aptamer nanoparticles, and also the chitosan-coated nanoparticles had more cytotoxic effects than the negatively charged albumin nanoparticles. The IC50 of targeted nanoparticles was 28 and 26% of free paclitaxel in MCF7 and T47D cells at 48h, respectively. Confocal laser scanning electron microscopy showed that aptamer conjugation and positive charge increase the cellular uptake. 66% of paclitaxel was released within 32h, but 100% of drug was released at pH=5.5 (similar cancer cells). The paclitaxel plasma amount was at a good level of 17.6% at 2h for increasing the chance of cellular uptake.
AuthorsMehdi Esfandyari-Manesh, Ali Mohammadi, Fatemeh Atyabi, Seyedeh Maryam Nabavi, Seyedeh Masoumeh Ebrahimi, Elnaz Shahmoradi, Behrang Shiri Varnamkhasti, Mohammad Hossein Ghahremani, Rassoul Dinarvand
JournalInternational journal of pharmaceutics (Int J Pharm) Vol. 515 Issue 1-2 Pg. 607-615 (Dec 30 2016) ISSN: 1873-3476 [Electronic] Netherlands
PMID27989825 (Publication Type: Journal Article)
CopyrightCopyright © 2016 Elsevier B.V. All rights reserved.
Chemical References
  • Aptamers, Nucleotide
  • Drug Carriers
  • MUC1 protein, human
  • Mucin-1
  • Serum Albumin
  • Chitosan
  • Paclitaxel
Topics
  • Aptamers, Nucleotide (administration & dosage, chemistry, genetics)
  • Breast Neoplasms (drug therapy, metabolism)
  • Cell Line, Tumor
  • Chitosan (administration & dosage, chemistry, pharmacokinetics)
  • Drug Carriers (administration & dosage, pharmacokinetics)
  • Female
  • Humans
  • MCF-7 Cells
  • Molecular Targeted Therapy
  • Mucin-1 (biosynthesis, genetics, metabolism)
  • Nanoparticles (administration & dosage, chemistry, metabolism)
  • Paclitaxel (pharmacology)
  • Serum Albumin (administration & dosage, chemistry, pharmacokinetics)

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