Poly(N-phenylglycine)-Based Nanoparticles as Highly Effective and Targeted Near-Infrared Photothermal Therapy/Photodynamic Therapeutic Agents for Malignant Melanoma.

Abstract

Malignant melanoma is a highly aggressive tumor resistant to chemotherapy. Therefore, the development of new highly effective therapeutic agents for the treatment of malignant melanoma is highly desirable. In this study, a new class of polymeric photothermal agents based on poly(N-phenylglycine) (PNPG) suitable for use in near-infrared (NIR) phototherapy of malignant melanoma is designed and developed. PNPG is obtained via polymerization of N-phenylglycine (NPG). Carboxylate functionality of NPG allows building multifunctional systems using covalent bonding. This approach avoids complicated issues typically associated with preparation of polymeric photothermal agents. Moreover, PNPG skeleton exhibits pH-responsive NIR absorption and an ability to generate reactive oxygen species, which makes its derivatives attractive photothermal therapy (PTT)/photodynamic therapy (PDT) dual-modal agents with pH-responsive features. PNPG is modified using hyaluronic acid (HA) and polyethylene glycol diamine (PEG-diamine) acting as the coupling agent. The resultant HA-modified PNPG (PNPG-PEG-HA) shows negligible cytotoxicity and effectively targets CD44-overexpressing cancer cells. Furthermore, the results of in vitro and in vivo experiments reveal that PNPG-PEG-HA selectively kills B16 cells and suppresses malignant melanoma tumor growth upon exposure to NIR light (808 nm), indicating that PNPG-PEG-HA can serve as a very promising nanoplatform for targeted dual-modality PTT/PDT of melanoma.

• Authors: Bang-Ping Jiang, Li Zhang, Xiao-Lu Guo, Xing-Can Shen, Yan Wang, Yang Zhu, Hong Liang
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 13 Issue 8 (02 2017) ISSN: 1613-6829 [Electronic] Germany
• PMID: 27982516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• N-phenylglycine
• Polyethylene Glycols
• Hyaluronic Acid
• Glycine

Topics

• Animals
• Cell Death (radiation effects)
• Cell Line, Tumor
• Glycine (analogs & derivatives, chemical synthesis, chemistry)
• Humans
• Hyaluronic Acid (chemical synthesis, chemistry)
• Hyperthermia, Induced
• Infrared Rays
• Melanoma (therapy)
• Melanoma, Experimental (pathology)
• Mice
• Microscopy, Atomic Force
• Nanoparticles (chemistry)
• Photochemotherapy
• Phototherapy
• Polyethylene Glycols (chemical synthesis, chemistry)
• Spectrophotometry, Ultraviolet
• Spectroscopy, Fourier Transform Infrared
• Thermogravimetry