Abstract | AIM: MATERIALS AND METHODS: RESULTS: Inhibitory effects of HL on the growth of WiDr cells were obtained along with apoptosis measurement. Selective accumulation of HL, including a fluorescence probe ( indocyanine green; (ICG)), was observed for WiDr cells using a fluorescence microscope. Remarkable reduction of tumor volume in xenograft model of mice topically HL-treated without drugs after the subcutaneous inoculation of WiDr cells was verified in vivo. Induction of apoptosis in tumor cells of xenograft mice topically administered with HL was observed in micrographs on the basis of terminal deoxynucleotidyl tranferase-mediated dUTP- biotin nick end labeling (TUNEL) method. Accumulation of HL, including ICG, for long-term into the tumor was obtained in the xenograft model. CONCLUSION:
Therapeutic effects of HL without any drugs in the murine xenograft model after subcutaneous inoculation of human colorectal cancer were revealed for the first time in vivo due to long-term accumulation.
|
Authors | Hideaki Ichihara, Masaki Okumura, Yoko Matsumoto |
Journal | Anticancer research
(Anticancer Res)
Vol. 36
Issue 11
Pg. 5875-5882
(11 2016)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 27793911
(Publication Type: Journal Article)
|
Copyright | Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
|
Topics |
- Animals
- Cell Line, Tumor
- Colorectal Neoplasms
(therapy)
- Humans
- Liposomes
- Mice
- Microscopy, Fluorescence
- Xenograft Model Antitumor Assays
|