Abstract | BACKGROUND: METHODS: We hypothesized that mesenchymal stromal cells (MSCs) would have beneficial effects in the treatment of H9N2 AIV-induced acute lung injury in mice. Six- to 8-week-old C57BL/6 mice were infected intranasally with 1 × 104 MID50 of A/HONG KONG/2108/2003 [H9N2 (HK)] H9N2 virus to induce acute lung injury. After 30 min, syngeneic MSCs were delivered through the caudal vein. Three days after infection, we measured the survival rate, lung weight, arterial blood gas, and cytokines in both bronchoalveolar lavage fluid (BALF) and serum, and assessed pathological changes to the lungs. RESULTS: MSC administration significantly palliated H9N2 AIV-induced pulmonary inflammation by reducing chemokines and proinflammatory cytokines levels, as well as reducing inflammatory cell recruit into the lungs. Thus, H9N2 AIV-induced lung injury was markedly alleviated in mice treated with MSCs. Lung histopathology and arterial blood gas analysis were improved in mice with H9N2 AIV-induced lung injury following MSC treatment. CONCLUSIONS:
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Authors | Yan Li, Jun Xu, Weiqing Shi, Cheng Chen, Yan Shao, Limei Zhu, Wei Lu, XiaoDong Han |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 7
Issue 1
Pg. 159
(10 28 2016)
ISSN: 1757-6512 [Electronic] England |
PMID | 27793190
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Acute Lung Injury
(therapy, virology)
- Animals
- Bronchoalveolar Lavage Fluid
- Chemokines
(metabolism)
- Female
- Influenza A Virus, H9N2 Subtype
- Lung
(pathology, virology)
- Mesenchymal Stem Cells
(cytology)
- Mice
- Mice, Inbred C57BL
- Orthomyxoviridae Infections
(therapy, virology)
- Pneumonia
(therapy, virology)
- Survival Rate
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