Abstract | PURPOSE:
Cofilin 1 is a type of cytoskeletal protein. The overexpression of this gene has been regarded to hold a special relationship with the development and progress of some cancers. However, the detailed position of Cofilin 1 in human bladder cancer has not been investigated intensively. METHODS: In this study, we mainly explored the relationship between human bladder cancer and the expression of Cofilin 1. The expression of Cofilin 1 in bladder cancer tissues and paracancerous tissues of patients was evaluated with quantitative polymerase chain reaction, Western blot, and immunohistochemical staining. Downregulation of Cofilin 1 expression model was established with siRNA in human RT4 bladder cancer cell line, and the changing cell viability was analyzed to determine the role of Cofilin 1 in human bladder cancer. RESULTS: Our results showed that the expression of Cofilin 1 was much higher in both RNA level and protein level in human bladder cancer tissues than paracancerous tissues for 3 patients. Downregulation of Cofilin 1 expression could inhibit cell proliferation, cell migration, cell adhesion, and colony formation ability, and increase the percentage of cell apoptosis in RT4 cells. CONCLUSIONS: Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer, and this gene might become a novel target in the diagnosis and treatment of human bladder cancer.
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Authors | Fei Wang, Dinglan Wu, Fengrong He, Housheng Fu, Jianbing Xu, Jianxiang Chen, Shufang Zhang, Weifu Wang |
Journal | Tumori
(Tumori)
Vol. 103
Issue 6
Pg. 537-542
(Nov 23 2017)
ISSN: 2038-2529 [Electronic] United States |
PMID | 27768223
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- CFL1 protein, human
- Cofilin 1
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Topics |
- Biomarkers, Tumor
(analysis)
- Cell Adhesion
(physiology)
- Cell Movement
(physiology)
- Cell Proliferation
(physiology)
- Cofilin 1
(biosynthesis)
- Humans
- Up-Regulation
- Urinary Bladder Neoplasms
(metabolism, pathology)
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