Abstract |
Nodal is a member of transforming growth factor beta (TGF-β) superfamily. Nodal promotes the self-renewal of human cancer stem cells (CSCs) and triggers carcinogenesis of human cancers via an autocrine manner through Smad2/3 pathway. In our study, generation of Nodal-overexpressed cancer cells was constructed, and the effect of Nodal on the stem cell marker Oct-4 was evaluated by overexpression or blocked Nodal/ALKs signaling pathway in non-small cell lung cancer cells A549 and prostate cancer cells PC3. Functionally, Nodal also increased the proliferation via the β- catenin nuclear translocation. This increase was attributed to GSK-3β dephosphorylating, and activin receptor-like kinase 4/7 (ALK4/7) played a major role in human cancer cells. Our study provides a positive understanding of Nodal function in cancer cells and suggests a potential novel target for clinical therapeutic research.
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Authors | Yi-Fei Qi, Long Wu, Zi-Qian Li, Meng-Ling Wu, Hai-Fang Wang, Ka-Ying Chan, Lin-Lin Lu, Shao-Hui Cai, Hong-Sheng Wang, Jun Du |
Journal | Archives of biochemistry and biophysics
(Arch Biochem Biophys)
Vol. 608
Pg. 34-41
(10 15 2016)
ISSN: 1096-0384 [Electronic] United States |
PMID | 27592306
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Chemical References |
- CTNNB1 protein, human
- NODAL protein, human
- Nodal Protein
- Octamer Transcription Factor-3
- POU5F1 protein, human
- beta Catenin
- ACVR1B protein, human
- Activin Receptors, Type I
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Topics |
- A549 Cells
- Active Transport, Cell Nucleus
- Activin Receptors, Type I
(metabolism)
- Cell Line, Tumor
- Cell Nucleus
(metabolism)
- Cell Proliferation
- Cytoplasm
(metabolism)
- Gene Expression Regulation, Neoplastic
- Humans
- Male
- Nodal Protein
(metabolism)
- Octamer Transcription Factor-3
(metabolism)
- Prostatic Neoplasms
(metabolism)
- Signal Transduction
- Transfection
- beta Catenin
(metabolism)
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