Inactivated coxsackievirus A10 experimental vaccines protect mice against lethal viral challenge.

Abstract

Coxsackievirus A10 (CVA10) has become one of the major causative agents of hand, foot and mouth disease (HFMD). It is now recognized that CVA10 should be targeted for vaccine development. We report here that β-propiolactone inactivated whole-virus based CVA10 vaccines can elicit protective immunity in mice. We prepared two inactivated CVA10 experimental vaccines derived from the prototype strain CVA10/Kowalik and from a clinical isolate CVA10/S0148b, respectively. Immunization with the experimental vaccines elicited CVA10-specific serum antibodies in mice. The antisera from vaccinated mice could potently neutralize in vitro infection with either homologous or heterologous CVA10 strains. Importantly, passive transfer of the anti-CVA10 sera protected recipient mice against CVA10/Kowalik or CVA10/S0148b infections. Moreover, active immunization with the inactivated vaccines also conferred protection against homologous and heterologous infections in mice. Collectively, our results demonstrate the proof-of-concept for inactivated whole-virus based CVA10 vaccines.

• Authors: Chaoyun Shen, Qingwei Liu, Yu Zhou, Zhiqiang Ku, Lili Wang, Ke Lan, Xiaohua Ye, Zhong Huang
• Journal: Vaccine (Vaccine) Vol. 34 Issue 41 Pg. 5005-5012 (09 22 2016) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 27562093 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Vaccines, Inactivated
• Viral Vaccines

Topics

• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Enterovirus
• Female
• Hand, Foot and Mouth Disease (prevention & control)
• Immunization, Passive
• Mice
• Mice, Inbred BALB C
• Mice, Inbred ICR
• Neutralization Tests
• Vaccines, Inactivated (immunology)
• Viral Vaccines (immunology)