Dextran sulfate sodium-induced acute experimental colitis in C57BL/6 mice is mitigated by selenium.

Abstract

BACKGROUND:

Sodium selenite has been shown to have a protective role in experimental colitis. Th1 and Th17 responses are involved in the pathogenesis of dextran sulfate sodium (DSS)-induced colitis and inflammatory bowel disease. This study investigated whether sodium selenite can suppress Th1/Th17-mediated experimental colitis.

METHODS:

Mice were administered sodium selenite (2μg/g body weight) by gavage daily for 30days. Beginning on day 21, mice were administered 2.5% oral DSS for 9days. The mice were sacrificed on day 31. Survival rates, clinical symptoms, colon lengths, and histological changes were determined.

RESULTS:

Pretreatment with sodium selenite (2μg/g body weight) improved survival rates, colon shortening, body weight loss, disease activity index, and histopathological score in mice with DSS-induced colitis. Pretreatment with sodium selenite restored interleukin-10 and Foxp3 excretion, as well as reducing the levels of interferon-γ and interleukin-17A.

CONCLUSIONS:

Pretreatment with sodium selenite showed therapeutic potential for preventing colitis in mice. This effect may be mediated by the immunomodulation of regulatory T cells, expressing anti-inflammatory genes that suppress Th1 and Th17 responses.

• Authors: Lixuan Sang, Bing Chang, Junfeng Zhu, Fangli Yang, Yan Li, Xuefeng Jiang, Xun Sun, Changlong Lu, Danan Wang
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 39 Pg. 359-368 (Oct 2016) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 27533281 (Publication Type: Journal Article)
• Copyright: Copyright © 2016. Published by Elsevier B.V.

Chemical References

• Forkhead Transcription Factors
• Foxp3 protein, mouse
• Interleukin-17
• Interleukin-10
• Interferon-gamma
• Dextran Sulfate
• Sodium Selenite

Topics

• Acute Disease
• Animals
• Colitis (chemically induced, drug therapy)
• Colon (drug effects, pathology)
• Dextran Sulfate
• Forkhead Transcription Factors (metabolism)
• Humans
• Interferon-gamma (metabolism)
• Interleukin-10 (metabolism)
• Interleukin-17 (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Models, Animal
• Sodium Selenite (therapeutic use)
• T-Lymphocytes, Regulatory (drug effects, immunology)
• Th1 Cells (immunology)
• Th17 Cells (immunology)