Abstract | BACKGROUND: METHODS:
AZD8055 (a new mTOR inhibitor)- or rapamycin-induced cytotoxic effects on neuroblastoma cells were studied. Western blotting was used to investigate the expression of various proteins in the mTOR pathway. MicroRNA precursors and antagomirs were transfected into cells to manipulate the expression of target microRNA. RESULTS: CONCLUSION:
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Authors | Yun Chen, Ya-Hui Tsai, Bor-Jiun Tseng, Hsin-Yen Pan, Sheng-Hong Tseng |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 51
Issue 11
Pg. 1818-1825
(Nov 2016)
ISSN: 1531-5037 [Electronic] United States |
PMID | 27492819
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Chemical References |
- Immunosuppressive Agents
- MIRN19 microRNA, human
- MicroRNAs
- Morpholines
- RNA, Neoplasm
- (5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol
- MTOR protein, human
- TOR Serine-Threonine Kinases
- Sirolimus
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Topics |
- Blotting, Western
- Cell Line, Tumor
- Gene Expression Regulation, Neoplastic
- Humans
- Immunosuppressive Agents
(pharmacology)
- MicroRNAs
(antagonists & inhibitors, genetics, metabolism)
- Morpholines
(pharmacology)
- Neuroblastoma
(genetics, metabolism, pathology)
- RNA, Neoplasm
(genetics)
- Real-Time Polymerase Chain Reaction
- Sirolimus
(pharmacology)
- TOR Serine-Threonine Kinases
(antagonists & inhibitors, metabolism)
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