The action and mechanism of myrislignan on A549 cells in vitro and in vivo.

Abstract

Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.

• Authors: XinGang Lu, Liu Yang, JingXian Chen, JiAn Zhou, XiaoDan Tang, YingGang Zhu, HongFu Qiu, Jie Shen
• Journal: Journal of natural medicines (J Nat Med) Vol. 71 Issue 1 Pg. 76-85 (Jan 2017) ISSN: 1861-0293 [Electronic] Japan
• PMID: 27491743 (Publication Type: Journal Article)

Chemical References

• 2-(4-allyl-2,6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)propan-1-ol
• Biological Products
• Lignans

Topics

• A549 Cells
• Apoptosis
• Biological Products (pharmacology, therapeutic use)
• Cell Line, Tumor
• Cell Proliferation
• Humans
• Immunohistochemistry
• Lignans (pharmacology, therapeutic use)
• Lung Neoplasms (drug therapy)
• Signal Transduction
• Xenograft Model Antitumor Assays