A preliminary study on the use of enzyme-linked immunosorbent assay (ELISA) for detection of Trichinella spiralis infections in dogs.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to detect Trichinella spiralis infections in dogs using rabbit anti-canine IgG-horseradish peroxidase prepared according to the improved periodate method and an antigen purified from T. spiralis larvae by Sephadex G-200 chromatography. Sixty-six canine sera were tested for trichinosis by the ELISA and it showed a detection rate which was significantly higher than that by trichinoscopy. This antigen of T. spiralis appeared not to cross-react with the sera of dogs infected with Ancylostoma caninum or Taenia spp. A comparison of ortho-phenylenediamine and 5-amino-2-hydroxybenzoic acid as substrates in the ELISA did not reveal a significant difference. Pieces of filter paper saturated with a defined quantity of whole blood can be substituted for serum as a source material for the test. The relationship between worm burden and the absorbance value in ELISA is discussed.

• Authors: S M Yang
• Journal: Veterinary parasitology (Vet Parasitol) Vol. 31 Issue 2 Pg. 165-71 (May 1989) ISSN: 0304-4017 [Print] Netherlands
• PMID: 2741302 (Publication Type: Journal Article)

Chemical References

• Antibodies, Helminth

Topics

• Animals
• Antibodies, Helminth (analysis)
• Dog Diseases (diagnosis)
• Dogs
• Enzyme-Linked Immunosorbent Assay
• Female
• Male
• Trichinella (immunology)
• Trichinellosis (diagnosis, veterinary)