Long non-coding RNA MINCR promotes gallbladder cancer progression through stimulating EZH2 expression.

Abstract

The regulation of MYC-regulated long non-coding RNAs has been reported to contribute to certain types of cancers. However, the role of MYC-induced long non-coding RNA (MINCR) in the tumorigenesis of gallbladder cancer (GBC) is still largely unknown. In this study, we discovered that MINCR was markedly upregulated in GBC tissues compared with adjacent normal tissues. High MINCR expression levels in GBC were positively associated with tumor volume and lymph node metastasis and were negatively correlated with overall survival (OS). Upregulation of MINCR and enhancer of zeste homolog 2 (EZH2) in GBC coincided with the downregulation of miR-26a-5p in GBC. Mechanistically, MINCR/miR-26a-5p/EZH2 axis was found to be involved in cell proliferation, cell invasive and apoptosis in GBC cells. Moreover, knockdown of MINCR suppressed cell proliferation, decreased S-phase cell numbers, increased cell apoptosis, and inhibited cell invasion by inhibiting the epithelial-mesenchymal transition (EMT) phenomenon in GBC cells. In vivo, tumor volumes were significantly decreased in the MINCR silencing group compared with those in the control group. These results demonstrated that MINCR could potentially be a therapeutic target as well as a prognostic marker in GBC.

• Authors: Shou-Hua Wang, Yong Yang, Xiao-Cai Wu, Ming-Di Zhang, Ming-Zhe Weng, Di Zhou, Jian-Dong Wang, Zhi-Wei Quan
• Journal: Cancer letters (Cancer Lett) Vol. 380 Issue 1 Pg. 122-33 (09 28 2016) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 27345740 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• MIRN26A microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• long non-coding RNA MINCR, human
• EZH2 protein, human
• Enhancer of Zeste Homolog 2 Protein

Topics

• Apoptosis
• Cell Cycle Checkpoints
• Cell Line
• Cell Movement
• Cell Proliferation
• Disease Progression
• Enhancer of Zeste Homolog 2 Protein (genetics, metabolism)
• Epithelial-Mesenchymal Transition
• Gallbladder Neoplasms (genetics, metabolism, mortality, surgery)
• Gene Expression Regulation, Neoplastic
• Humans
• Lymphatic Metastasis
• MicroRNAs (genetics, metabolism)
• RNA Interference
• RNA, Long Noncoding (genetics, metabolism)
• Signal Transduction
• Time Factors
• Transfection
• Tumor Burden
• Up-Regulation