β-amyloid increases neurocan expression through regulating Sox9 in astrocytes: A potential relationship between Sox9 and chondroitin sulfate proteoglycans in Alzheimer's disease.

Abstract

OBJECTIVE:

This study aimed to investigate whether β-amyloid (Aβ) was able to enhance neurocan expression in a Sox9 dependent manner in astrocytes.

METHODS AND MATERIALS:

Astrocytes were incubated with Aβ at different concentrations, the expression of Sox9 and neurocan was detected by Western blot assay. Meanwhile, the viability and proliferation of astrocytes were assessed by MTT assay. Then, the Sox9 expression was silenced, and the expression of Sox9 and neurocan was examined.

RESULTS:

After incubation with Aβ, the viability of astrocytes was increased regardless silencing of Sox9 (all P<0.05). The proliferation of astrocytes was also gradually increased with the increase in the time of Aβ incubation (all P<0.05). With the increase in Aβ concentration, the expression of Sox9 and neurocan was also increased (all P<0.05). However, after silencing of Sox9 expression, the neurocan expression was significantly reduced as compared to control group and scra-siRNA group (all P<0.05).

CONCLUSION:

Our study shows the viability and proliferation of astrocytes are significantly increased by Aβ in a dose dependent manner. Moreover, Aβ may effectively up-regulate the neurocan expression via regulating Sox9.

• Authors: Han Yan, Xiaolong Zhu, Junchao Xie, Yanxin Zhao, Xueyuan Liu
• Journal: Brain research (Brain Res) Vol. 1646 Pg. 377-383 (09 01 2016) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 27317830 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Chondroitin Sulfate Proteoglycans
• Neurocan
• Peptide Fragments
• RNA, Messenger
• SOX9 Transcription Factor
• Sox9 protein, mouse
• amyloid beta-protein (1-42)

Topics

• Alzheimer Disease (metabolism)
• Amyloid beta-Peptides (administration & dosage)
• Animals
• Astrocytes (metabolism)
• Cell Proliferation
• Cell Survival
• Cells, Cultured
• Chondroitin Sulfate Proteoglycans (metabolism)
• Mice
• Mice, Inbred C57BL
• Neurocan (metabolism)
• Peptide Fragments (administration & dosage)
• RNA, Messenger (metabolism)
• SOX9 Transcription Factor (metabolism)