Evaluation of immunomodulatory effect of recombinant human granulocyte-macrophage colony-stimulating factor on polymorphonuclear cell from dogs with cancer in vitro.

Abstract

The objective of this in vitro study was to evaluate the immunomodulatory effects of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on polymorphonuclear cell (PMN) function in dogs with cancer. PMNs were harvested from dogs with naturally developing cancer as a pre-clinical model to evaluate the immunomodulatory effects of rhGM-CSF on PMN phagocytic and cytotoxic functions, cytokine production and receptor expression. Some aspects of cancer-related PMN dysfunction in dogs with cancer were restored following incubation with rhGM-CSF including PMN phagocytosis, respiratory burst and LPS-induced TNF-α production. In addition, rhGM-CSF increased surface HLA-DR expression on the PMNs of dogs with cancer. These data suggests that dysfunction of innate immune response in dogs with cancer may be improved by rhGM-CSF. The results of this study provided a pathophysiologic rationale for the initiation of clinical trials to continue evaluating rhGM-CSF as an immunomodulatory therapy in dogs with cancer.

• Authors: Y Zhang, S Axiak-Bechtel, C Friedman Cowan, J Amorim, K Tsuruta, A E DeClue
• Journal: Veterinary and comparative oncology (Vet Comp Oncol) Vol. 15 Issue 3 Pg. 968-979 (Sep 2017) ISSN: 1476-5829 [Electronic] England
• PMID: 27098709 (Publication Type: Journal Article)
• Copyright: © 2016 John Wiley & Sons Ltd.

Chemical References

• Immunologic Factors
• Recombinant Proteins
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Animals
• Dog Diseases (drug therapy)
• Dogs
• Female
• Granulocyte-Macrophage Colony-Stimulating Factor (pharmacology)
• Immunologic Factors (therapeutic use)
• In Vitro Techniques
• Male
• Neoplasms (drug therapy, veterinary)
• Neutrophils (drug effects)
• Recombinant Proteins