Topical formulation containing hesperidin methyl chalcone inhibits skin oxidative stress and inflammation induced by ultraviolet B irradiation.

Abstract

Skin exposure to ultraviolet B (UVB) irradiation has increased significantly in recent years due to ozone depletion, and it represents the main cause of many skin diseases. Hesperidin methyl chalcone (HMC) is a compound used to treat vascular diseases that has demonstrated anti-inflammatory activities in pre-clinical studies. Herein, we tested the antioxidant activity of HMC in cell free systems and the in vivo effects of a stable topical formulation containing HMC in a mouse model of skin oxidative stress and inflammation induced by UVB irradiation. HMC presented ferric reducing power, neutralized 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radicals, and inhibited lipid peroxidation. In hairless mice, a topical formulation containing HMC inhibited UVB irradiation-induced skin edema, depletion of antioxidant capacity (ferric and ABTS reducing abilities and catalase activity), lipid peroxidation, superoxide anion production and mRNA expression of gp91phox (nicotinamide adenine dinucleotide phosphate [NADPH] oxidase 2 sub-unity). In addition, HMC inhibited UVB irradiation-induced depletion of reduced glutathione levels by maintaining glutathione peroxidase-1 and glutathione reductase mRNA expression, prevented down-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression and increased heme oxygenase-1 mRNA expression. Finally, we demonstrated that topical application of the formulation containing HMC inhibited cytokine (TNF-α, IL-1β, IL-6, and IL-10) production induced by UVB irradiation. Therefore, this topical formulation containing HMC is a promising new therapeutic approach to protecting the skin from the deleterious effects of UVB irradiation.

• Authors: Renata M Martinez, Felipe A Pinho-Ribeiro, Vinicius S Steffen, Carla V Caviglione, Danilo Pala, Marcela M Baracat, Sandra R Georgetti, Waldiceu A Verri, Rubia Casagrande
• Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 15 Issue 4 Pg. 554-63 (Apr 2016) ISSN: 1474-9092 [Electronic] England
• PMID: 27021784 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chalcones
• Cytokines
• Membrane Glycoproteins
• NF-E2-Related Factor 2
• Nfe2l2 protein, rat
• RNA, Messenger
• Superoxides
• hesperidin methylchalcone
• Hesperidin
• Heme Oxygenase-1
• Cybb protein, mouse
• NADPH Oxidase 2
• NADPH Oxidases

Topics

• Administration, Topical
• Animals
• Chalcones (administration & dosage, pharmacology)
• Cytokines (biosynthesis)
• Heme Oxygenase-1 (genetics)
• Hesperidin (administration & dosage, analogs & derivatives, pharmacology)
• Inflammation (metabolism, prevention & control)
• Membrane Glycoproteins (genetics, metabolism)
• Mice
• Mice, Hairless
• NADPH Oxidase 2
• NADPH Oxidases (genetics, metabolism)
• NF-E2-Related Factor 2 (genetics)
• Oxidative Stress (drug effects)
• RNA, Messenger (genetics)
• Skin (drug effects, metabolism)
• Superoxides (metabolism)
• Ultraviolet Rays