Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells.

Abstract

Three-dimensional (3D) cell culture is a powerful tool to study cell growth under 3D condition. To perform a simple test for anti-cancer drugs in 3D culture, visualization of non-proliferated cells is required. We propose a fluorescent imaging-based assay to analyze cancer cell proliferation in 3D culture. We used a pulse-labeling technique with a photoconvertible fluorescent protein Kaede to identify non-proliferated cells. This assay allows us to observe change in cell proliferation in 3D culture by simple imaging. Using this assay, we obtained the data of the effects of anti-cancer drugs, 5-fluorouracil and PD0332991 in a breast cancer cell line, MCF-7.

• Authors: Junji Itou, Sunao Tanaka, Wenzhao Li, Yoshiaki Matsumoto, Fumiaki Sato, Masakazu Toi
• Journal: Data in brief (Data Brief) Vol. 5 Pg. 429-33 (Dec 2015) ISSN: 2352-3409 [Print] Netherlands
• PMID: 26958605 (Publication Type: Journal Article)