Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma.

Abstract

Long non-coding RNAs (lncRNA) have been shown to play important roles in the development and progression of cancer. Here, we discovered a novel long noncoding RNA (lncRNA) FEZF1 antisense RNA1 (FEZF1-AS1) is markedly upregulated in human primary colorectal carcinoma (CRC) and associated with CRC metastasis and poor prognosis. Moreover, the downregulation of FEZF1-AS1 expression significantly inhibited the CRC cells proliferation, migration and invasiveness, suppressed S-phase entry in vitro, and repressed tumor growth and metastasis in vivo. In contrast, overexpression of FEZF1-AS1 could promote the aggressive behaviors of CRC cells. We further discovered that the downregulation of FEZF1-AS1 reduced its sense-cognate gene FEZF1 mRNA and protein expression in CRC cells. There was a positive correlation between FEZF1-AS1 and FEZF1 expression in CRC. Moreover, FEZF1 knockdown also significantly suppressed CRC cell proliferation, migration, and invasion. Our findings indicate that the dysregulation of FEZF1-AS1 participates in colorectal tumorigenesis and progression, which might be achieved, at least in part, through FEZF1 induction.

• Authors: Na Chen, Dan Guo, Qiong Xu, Minhui Yang, Dan Wang, Man Peng, Yanqing Ding, Shuang Wang, Jun Zhou
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 10 Pg. 11271-83 (Mar 08 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 26848625 (Publication Type: Journal Article)

Chemical References

• FEZF1 protein, human
• RNA, Antisense
• RNA, Messenger
• RNA, Untranslated
• Repressor Proteins
• Transcription Factors

Topics

• Animals
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Female
• Gene Knockdown Techniques
• HCT116 Cells
• HEK293 Cells
• HT29 Cells
• Heterografts
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Prognosis
• RNA, Antisense (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)
• RNA, Untranslated (biosynthesis, genetics)
• Repressor Proteins
• Transcription Factors (biosynthesis, genetics)