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Two subunits of mannose permease, II-PMan and II-MMan, of Escherichia coli mediate coliphage N4 infection.

Abstract
Mutant strains of Escherichia coli that lack one or all of the intact components of mannose permease do not support the growth of phage N4. Complementation experiments using three recombinant plasmids containing DNA fragments coding for the subunits of mannose permease revealed that among the three component subunits, II-PMan and II-MMan alone are sufficient to confer N4 sensitivity.
AuthorsK Kim, O J Yoo
JournalBiochemistry international (Biochem Int) Vol. 18 Issue 3 Pg. 545-9 (Mar 1989) ISSN: 0158-5231 [Print] Australia
PMID2669760 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • DNA, Recombinant
  • Recombinant Proteins
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • phosphoenolpyruvate-mannose phosphotransferase
Topics
  • Coliphages (genetics, pathogenicity)
  • DNA, Recombinant
  • Escherichia coli (enzymology, genetics)
  • Genetic Code
  • Genetic Complementation Test
  • Mutation
  • Phosphoenolpyruvate Sugar Phosphotransferase System (genetics, metabolism)
  • Plasmids
  • Recombinant Proteins (metabolism)
  • Suppression, Genetic

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