Serum cyclin-dependent kinase 9 is a potential biomarker of atherosclerotic inflammation.

Abstract

Atherosclerotic coronary artery disease (CAD) is one of the most prevalent diseases worldwide. Atherosclerosis was considered to be the single most important contributor to CAD. In this study, a distinct serum protein expression pattern in CAD patients was demonstrated by proteomic analysis with two-dimensional gel electrophoresis coupled with mass spectrometry. In particular, CDK9 was found to be highly elevated in serum, monocytes and artery plaque samples of CAD patients. Furthermore, there was high infiltration of CD14+ monocytes/macrophages within artery plaques correlated with the expression of CDK9. Moreover, Flavopiridol (CDK9 inhibitor) could inhibit THP-1 cell (monocytic acute leukemia cell line) proliferation by targeting CDK9. Altogether, These findings indicate that CDK9 represent an important role for inflammation in the pathogenesis of atherosclerosis. It may be a potential biomarker of atherosclerotic inflammation and offer insights into the pathophysiology and targeted therapy for atherosclerotic CAD.

• Authors: Yeming Han, Shanshan Zhao, Yaoqin Gong, Guihua Hou, Xi Li, Li Li
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 2 Pg. 1854-62 (Jan 12 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 26636538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Flavonoids
• Piperidines
• Protein Kinase Inhibitors
• alvocidib
• Cyclin-Dependent Kinase 9

Topics

• Aged
• Apoptosis (drug effects)
• Atherosclerosis (blood, metabolism)
• Biomarkers (blood, metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cells, Cultured
• Coronary Artery Disease (blood, metabolism)
• Cyclin-Dependent Kinase 9 (blood, genetics, metabolism)
• Female
• Flavonoids (pharmacology)
• Gene Expression (drug effects)
• Humans
• Immunohistochemistry
• Inflammation (blood, metabolism)
• Male
• Middle Aged
• Monocytes (drug effects, metabolism)
• Piperidines (pharmacology)
• Protein Kinase Inhibitors (pharmacology)
• Proteomics (methods)
• Reverse Transcriptase Polymerase Chain Reaction