Sodium tanshinone IIA sulfonate affects Marek's disease virus replication by inhibiting gB expression.

Abstract

CONTEXT:

Previous studies demonstrated that sodium tanshinone IIA sulfonate (STS) could inhibit MDV replication in vitro. The mechanism about how STS inhibits MDV replication is still not well understood.

OBJECTIVE:

In this study, we evaluated the effect of STS on gB gene/protein of Marek's disease virus (MDV).

MATERIALS AND METHODS:

The concentration of 0.25 mg/ml of STS was used in this study. Meanwhile, 0.25 mg/ml of acyclovir (ACV) was used as a positive control. About 9-11-d-old embryonated specific-pathogen-free (SPF) chicken eggs were used to prepare CEF cells. CEF cells were infected with MDV 2 h, followed by treatment with STS. Real-time PCR and western blot assay were used to measure the gB (UL27) gene/protein expression in STS treatment group at 24, 48, 72, and 96 h post-infection.

RESULTS:

Compared with MDV control, the gB gene copies were significantly decreased in STS and ACV treatment groups at 72 h and 96 h (p < 0.05), both in the DNA and in the mRNA level. Furthermore, the expression of gB protein was also inhibited by STS at 24, 72, and 96 h.

DISCUSSION AND CONCLUSION:

Our study demonstrated that STS could effectively inhibit the MDV replication by suppressing gB gene/protein expression in cell culture.

• Authors: Jin Wang, Panpan Sun, Yuning Feng, Jianhua Guo, Yaogui Sun, Haimin Lei, Jianqin Xu, Hongquan Li
• Journal: Pharmaceutical biology (Pharm Biol) Vol. 54 Issue 4 Pg. 701-4 ( 2016) ISSN: 1744-5116 [Electronic] England
• PMID: 26428057 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Phenanthrenes
• Viral Envelope Proteins
• glycoprotein B, Marek's disease virus
• tanshinone II A sodium sulfonate

Topics

• Animals
• Antigens, Viral (biosynthesis, genetics)
• Cells, Cultured
• Chick Embryo
• Gene Expression Regulation, Viral (drug effects, physiology)
• Marek Disease (genetics, metabolism)
• Phenanthrenes (pharmacology)
• Viral Envelope Proteins (antagonists & inhibitors, biosynthesis, genetics)
• Virus Replication (drug effects, physiology)