Nanostructured lipid carriers based temozolomide and gene co-encapsulated nanomedicine for gliomatosis cerebri combination therapy.

Abstract

BACKGROUND:

Co-delivery of gene and anticancer drug into the same cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment. In this study, nanostructured lipid carriers (NLCs) were constructed as multifunctional nanomedicine for co-delivery of enhanced green fluorescence protein plasmid (DNA) and temozolomide (TMZ).

METHODS:

TMZ- and DNA-loaded NLCs (TMZ/DNA-NLCs) were prepared. Their particle size, zeta potential, gene-loading capacity (GL) and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study TMZ/DNA-NLCs was tested in U87 malignant glioma cells (U87 MG cells). In vivo gene transfection and anti-tumor efficacy of the carriers were evaluated on mice bearing malignant glioma model.

RESULTS:

The optimum TMZ/DNA-NLCs formulations with the particle size of 179 nm and with a +23 mV surface charge; got 91% of GL and 83% of EE. The growth of U87 MG cells in vitro was obviously inhibited. TMZ/DNA-NLCs also displayed the highest gene transfection efficiency and the best antitumor activity than other formulations in vivo.

CONCLUSION:

The results demonstrated that TMZ/DNA-NLCs were efficient in selective delivery to malignant glioma cells. Also TMZ/DNA-NLCs transfer both drug and gene to the gliomatosis cerebri, enhance the antitumor capacity and gene transfection efficacy. Thus, TMZ/DNA-NLCs could prove to be a superior co-delivery nanomedicine to achieve therapeutic efficacy and this report could be a new promising strategy for treatment in malignant gliomatosis cerebri.

• Authors: Zhihua Chen, Xianliang Lai, Shuxin Song, Xingen Zhu, Jianming Zhu
• Journal: Drug delivery (Drug Deliv) Vol. 23 Issue 4 Pg. 1369-73 (May 2016) ISSN: 1521-0464 [Electronic] England
• PMID: 26017099 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Drug Carriers
• Lipids
• Dacarbazine
• DNA
• Temozolomide

Topics

• Animals
• Antineoplastic Agents (chemistry, metabolism, pharmacology)
• Cell Line, Tumor
• Chemistry, Pharmaceutical
• DNA (chemistry, genetics, pharmacology)
• Dacarbazine (analogs & derivatives, chemistry, metabolism, pharmacology)
• Drug Carriers
• Drug Delivery Systems
• Humans
• Lipids (chemistry)
• Mice
• Nanomedicine
• Nanostructures
• Neoplasms, Neuroepithelial (chemistry, drug therapy)
• Temozolomide
• Transfection