Collagen XVII expression correlates with the invasion and metastasis of colorectal cancer.

Abstract

Collagen XVII has a well-established role as an adhesion molecule and a cell surface receptor located in the type I hemidesmosome of stratified epithelia. Its ectodomain is constitutively shed from the cell surface and suggested to regulate the adhesion, migration, and signaling of cutaneous epithelial cells. Collagen XVII was not previously thought to be expressed by colon epithelial cells. Immunohistochemical analysis of tissue microarray samples of 141 cases of colorectal carcinoma showed that collagen XVII is expressed in normal human colonic mucosa and colorectal carcinoma. In colorectal carcinoma, increased collagen XVII expression was significantly associated with higher TNM stage. It also correlated with infiltrative growth pattern and tumor budding as well as lymph node and distant metastasis. Increased collagen XVII expression was associated with decreased disease-free and cancer-specific survival. Immunofluorescence staining of collagen XVII and its well-known binding partner laminin γ2 chain demonstrated a partial colocalization in normal and tumor tissue. In vitro, the overexpression of murine collagen XVII promoted the invasion of CaCo-2 colon carcinoma cells through Matrigel (BD Biosciences; Bedford, MA). To conclude, this study reports for the first time the expression of collagen XVII in colon epithelium and the association of increased collagen XVII immunoexpression with poor outcome in colorectal carcinoma.

• Authors: Jyri M Moilanen, Nina Kokkonen, Stefanie Löffek, Juha P Väyrynen, Erkki Syväniemi, Tiina Hurskainen, Markus Mäkinen, Kai Klintrup, Jyrki Mäkelä, Raija Sormunen, Leena Bruckner-Tuderman, Helena Autio-Harmainen, Kaisa Tasanen
• Journal: Human pathology (Hum Pathol) Vol. 46 Issue 3 Pg. 434-42 (Mar 2015) ISSN: 1532-8392 [Electronic] United States
• PMID: 25623077 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Autoantigens
• Biomarkers, Tumor
• Non-Fibrillar Collagens
• collagen type XVII

Topics

• Aged
• Autoantigens (analysis)
• Basement Membrane (chemistry, pathology)
• Biomarkers, Tumor (analysis)
• Caco-2 Cells (chemistry, pathology)
• Colorectal Neoplasms (chemistry, mortality, pathology, therapy)
• Disease-Free Survival
• Female
• Fluorescent Antibody Technique
• Follow-Up Studies
• Humans
• Immunohistochemistry
• Intestinal Mucosa (chemistry)
• Male
• Middle Aged
• Neoplasm Invasiveness (pathology)
• Neoplasm Staging
• Neoplasms, Squamous Cell (chemistry, mortality, pathology, secondary, therapy)
• Non-Fibrillar Collagens (analysis)
• ROC Curve
• Survival Analysis
• Treatment Outcome