Abstract |
Tetanus toxin labeling of human lung cancer cell lines was investigated using direct and indirect immunofluorescence and immunohistochemical staining. Cells of characterized permanent cell lines, eight small-cell lung cancer (SCLC) cell lines of classic subtype, six SCLC cell lines of variant subtype and seven non-small-cell lung cancer (NSCLC) cell lines, were incubated with a saturating concentration of tetanus toxin. For staining, fluorescein- isothiocyanate-( FITC)-conjugated anti-( tetanus toxin) antibodies were used or a mouse monoclonal anti-(fragment C) antibody with subsequent binding of FITC-conjugated anti-(mouse Ig) antibody or peroxidase-anti- peroxidase complex. Only SCLC showed an intense fluorescence/immunoreactivity restricted to the cell membrane. Quantitative analysis of tetanus toxin labeling by flow cytometry revealed the percentage of positive cells to be between 35% and 95% in SCLC without obvious differences between the classic and variant subtypes of SCLC. In NSCLC the percentage of positive cells was lower than 10%. These results demonstrate that SCLC in contrast to NSCLC can be labeled with tetanus toxin, emphasizing the neuroendocrine properties of this tumor, and that tetanus toxin labeling may become a useful diagnostic marker for SCLC cells in cytology.
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Authors | J Heymanns, K Neumann, K Havemann |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 115
Issue 6
Pg. 537-42
( 1989)
ISSN: 0171-5216 [Print] Germany |
PMID | 2558117
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Tetanus Toxoid
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Topics |
- Animals
- Biomarkers, Tumor
(analysis)
- Carcinoma, Small Cell
(diagnosis)
- Flow Cytometry
- Fluorescent Antibody Technique
- Lung Neoplasms
(diagnosis)
- Mice
- Mice, Nude
- Microscopy, Fluorescence
- Neoplasm Transplantation
- Tetanus Toxoid
- Tumor Cells, Cultured
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