GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes.

Abstract

INTRODUCTION:

In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal-epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124-131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.

MATERIALS AND METHODS:

Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.

RESULTS:

We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.

CONCLUSIONS:

Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.

• Authors: L N Guerra, C Suarez, D Soto, A Schiappacasse, D Sapochnik, P Sacca, G Piwien-Pilipuk, B Peral, J C Calvo
• Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (Clin Transl Oncol) Vol. 17 Issue 7 Pg. 511-20 (Jul 2015) ISSN: 1699-3055 [Electronic] Italy
• PMID: 25532485 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCAAT-Enhancer-Binding Protein-beta
• Fabp4 protein, mouse
• Fatty Acid-Binding Proteins
• Triglycerides
• Sulfurtransferases
• GAL3ST2 protein, human
• Gal3St2 protein, mouse
• Sulfotransferases

Topics

• 3T3-L1 Cells
• Adipocytes (cytology, metabolism)
• Adipogenesis
• Animals
• CCAAT-Enhancer-Binding Protein-beta (metabolism)
• Cell Differentiation
• Cell Line, Tumor
• Fatty Acid-Binding Proteins (metabolism)
• Female
• HEK293 Cells
• Humans
• MCF-7 Cells
• Mammary Glands, Animal (cytology, metabolism)
• Mice
• NIH 3T3 Cells
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Sulfotransferases (metabolism)
• Sulfurtransferases (metabolism)
• Triglycerides (metabolism)