Recombinant outer membrane protein A fragments protect against Escherichia coli meningitis.

Abstract

BACKGROUND:

Although the mortality rates have decreased over the past few decades, neonatal meningitis is still a severe disease with high morbidity. Moreover, approximately 40% of survivors exhibit neurological sequelae. Escherichia coli is the major Gram-negative bacterial pathogen in neonatal meningitis. The N-terminal β-barrel domain of the outer membrane protein A (OmpA) of E. coli is essential for effective protein conformation and function and contains four surface-exposed hydrophilic loops. In this study, we expressed different fragments of the four ring structures of the N-terminal domain, and investigated whether these recombinant OmpA fragments can protect mice from death after E. coli infection.

METHODS:

We expressed the recombinant proteins of the following OmpA fragments by using molecular cloning of Loop 1-2, Loop 1-3, Loop 1-4, Loop 2-3, Loop 2-4, and Loop 3-4. Animal experiments were subsequently performed to investigate the effects of these recombinant OmpA fragments on the survival of C57BL/6 mice after intracerebral E. coli RS218 administration.

RESULTS:

This study demonstrated that the recombinant Loop 1-3, Loop 2-3, and Loop 2-4 fragments of OmpA can protect mice from intracerebral E. coli infection.

CONCLUSION:

In bacterial meningitis, although antibiotic therapy is the first choice for management, neurological complications can seldom be averted. Based on the results of the present study, we intend to establish an effective therapeutic application for E. coli meningitis.

• Authors: Wen-Shyang Hsieh, Yi-Yuan Yang, Hsin-Yi Yang, Yu-Shan Huang, Hsueh-Hsia Wu
• Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (J Microbiol Immunol Infect) Vol. 49 Issue 3 Pg. 329-34 (Jun 2016) ISSN: 1995-9133 [Electronic] England
• PMID: 25305709 (Publication Type: Journal Article)
• Copyright: Copyright © 2014. Published by Elsevier B.V.

Chemical References

• Bacterial Outer Membrane Proteins
• Recombinant Proteins
• OMPA outer membrane proteins

Topics

• Animals
• Bacterial Outer Membrane Proteins (genetics, immunology)
• Escherichia coli (genetics, immunology)
• Escherichia coli Infections (immunology, microbiology, prevention & control)
• Humans
• Meningitis, Escherichia coli (microbiology, prevention & control)
• Mice
• Mice, Inbred C57BL
• Protein Structure, Tertiary
• Recombinant Proteins (immunology)