Comparative proteomic analysis of normal and
cancer cell lines provides for a better understanding of the molecular mechanism of
cancer development and is essential for developing more effective strategies for new
biomarker or
drug target discovery. The purpose of this study is to compare
protein expression levels between
fibrosarcoma and fibroblast cell lines. In our study, two-dimensional
polyacrylamide gel electrophoresis (2-D PAGE) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques were carried out to compare the
protein profile between
fibrosarcoma and fibroblast cell lines. We prepared cell lysate samples to analyze intracellular
proteins and secretome samples to analyze extracellular
proteins in both cell lines. Our results revealed 13 upregulated
proteins and 1 downregulated
protein of which all of them identified in
fibrosarcoma cell line after the comparison with fibroblast cell line cell lysates. When comparing secretome profiles of both cell lines, we found and identified 13
proteins only expressed in
fibrosarcoma cell line. These identified
proteins have common functions such as cell proliferation, cell differentiation, invasion,
metastasis, and apoptosis in
cancer. The data obtained from this study indicates that these
proteins have importance on understanding the molecular mechanism of
fibrosarcoma. These
proteins may serve as candidate
biomarkers and
drug targets for future clinical studies.