Bacterial growth kinetics: modelling and evaluation of two-compartment radioassay.

Abstract

Quantitative measurements of bacterial growth may be made using a radioassay technique. This method measures, by scintillation counting, the 14CO2 derived from the bacterial metabolism of a 14C-labeled substrate. Mathematical growth models may serve as reliable tools for estimation of the generation rate constant (or slope of the growth curve) and provide a basis for evaluating assay performance. Two models, i.e., exponential and logistic, are proposed. Both models yielded an accurate fit to the data from radioactive measurement of bacterial growth. The exponential model yielded high precision values of the generation rate constant, with an average relative standard deviation of 1.2%. Under most conditions the assay demonstrated no changes in the slopes of growth curves when the number of bacteria per inoculation was changed. However, the radiometric assay by scintillation method had a growth-inhibiting effect on a few strains of bacteria. The source of this problem was thought to be hypersensitivity to trace amounts of toluene remaining on the detector.

• Authors: V Boonkitticharoen, J C Ehrhardt, P T Kirchner
• Journal: Canadian journal of microbiology (Can J Microbiol) Vol. 35 Issue 9 Pg. 874-80 (Sep 1989) ISSN: 0008-4166 [Print] Canada
• PMID: 2509054 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Carbon Dioxide
• Carbon

Topics

• Bacteria (growth & development, metabolism)
• Carbon (metabolism)
• Carbon Dioxide (analysis)
• Culture Media
• Kinetics
• Models, Biological
• Regression Analysis
• Scintillation Counting