Abstract | BACKGROUND: METHODS: Thirty sinus tissue specimens were intraoperatively collected from 3 patient groups: AFRS; CRS without nasal polyps (CRSsNP); and controls (n = 10 per group). Specimens were analyzed by semiquantitative reverse-transcription polymerase chain reaction (sq-RT-PCR) and immunofluorescence (IF) labeling/confocal microscopy for the presence of both periostin and RANKL. Immunofluorescence staining intensity was quantified by pixel density analysis. Preoperative computed tomography (CT) scans from each patient were scored using both the Lund-Mackay and CT bone erosion scoring systems. RESULTS:
Periostin was significantly elevated in AFRS sinus tissue compared to CRSsNP and controls, as demonstrated by IF (p < 0.001) and PCR (p = 0.011). RANKL was not detected in sinus tissue by IF or PCR. Periostin levels positively correlated with radiographic indices of disease severity for both soft tissue and bone, using Lund-Mackay (r = 0.926 [PCR] and r = 0.581 [IF]) and CT bone erosion (r = 0.672 [PCR] and r = 0.616 [IF]) scoring systems, respectively. CONCLUSION:
Periostin is increased in AFRS tissue compared to CRSsNP and controls. Periostin levels positively correlate with radiologic disease severity scores. The increased levels of periostin in AFRS are possibly tied to its intense eosinophilic inflammatory etiology.
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Authors | Adrienne M Laury, Roland Hilgarth, Asma Nusrat, Sarah K Wise |
Journal | International forum of allergy & rhinology
(Int Forum Allergy Rhinol)
Vol. 4
Issue 9
Pg. 716-24
(Sep 2014)
ISSN: 2042-6984 [Electronic] United States |
PMID | 25060295
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 ARS-AAOA, LLC. |
Chemical References |
- Cell Adhesion Molecules
- POSTN protein, human
- RANK Ligand
- RNA, Messenger
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Topics |
- Adult
- Aged
- Cell Adhesion Molecules
(genetics, metabolism)
- Female
- Humans
- Male
- Middle Aged
- Mycoses
(diagnostic imaging, metabolism)
- Nasal Polyps
(diagnostic imaging, metabolism)
- RANK Ligand
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Rhinitis, Allergic
(diagnostic imaging, metabolism)
- Severity of Illness Index
- Sinusitis
(diagnostic imaging, metabolism)
- Tomography, X-Ray Computed
- Young Adult
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