The effect of superparamagnetic iron oxide with iRGD peptide on the labeling of pancreatic cancer cells in vitro: a preliminary study.

Abstract

The iRGD peptide loaded with iron oxide nanoparticles for tumor targeting and tissue penetration was developed for targeted tumor therapy and ultrasensitive MR imaging. Binding of iRGD, a tumor homing peptide, is mediated by integrins, which are widely expressed on the surface of cells. Several types of small molecular drugs and nanoparticles can be transfected into cells with the help of iRGD peptide. Thus, we postulate that SPIO nanoparticles, which have good biocompatibility, can also be transfected into cells using iRGD. Despite the many kinds of cell labeling studies that have been performed with SPIO nanoparticles and RGD peptide or its analogues, only a few have applied SPIO nanoparticles with iRGD peptide in pancreatic cancer cells. This paper reports our preliminary findings regarding the effect of iRGD peptide (CRGDK/RGPD/EC) combined with SPIO on the labeling of pancreatic cancer cells. The results suggest that SPIO with iRGD peptide can enhance the positive labeling rate of cells and the uptake of SPIO. Optimal functionalization was achieved with the appropriate concentration or concentration range of SPIO and iRGD peptide. This study describes a simple and economical protocol to label panc-1 cells using SPIO in combination with iRGD peptide and may provide a useful method to improve the sensitivity of pancreatic cancer imaging.

• Authors: Hou Dong Zuo, Wei Wu Yao, Tian Wu Chen, Jiang Zhu, Juan Juan Zhang, Yu Pu, Gang Liu, Xiao Ming Zhang
• Journal: BioMed research international (Biomed Res Int) Vol. 2014 Pg. 852352 ( 2014) ISSN: 2314-6141 [Electronic] United States
• PMID: 24977163 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Dextrans
• Magnetite Nanoparticles
• ferumoxtran-10
• GTP-Binding Proteins
• IRGM protein, human

Topics

• Cell Line, Tumor
• Contrast Media (chemical synthesis, pharmacokinetics)
• Dextrans (chemistry)
• GTP-Binding Proteins (chemistry, pharmacokinetics)
• Humans
• Magnetic Resonance Imaging (methods)
• Magnetite Nanoparticles (chemistry)
• Pancreatic Neoplasms (metabolism, pathology)
• Pilot Projects
• Reproducibility of Results
• Sensitivity and Specificity
• Staining and Labeling (methods)