Corilagin attenuates aerosol bleomycin-induced experimental lung injury.

Abstract

Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury following bleomycin exposure in an animal model of pulmonary fibrosis. Corilagin abrogated bleomycin-induced lung fibrosis as assessed by H&E; Masson's trichrome staining and lung hydroxyproline content in lung tissue. Corilagin reduced the number of apoptotic lung cells and prevented lung epithelial cells from membrane breakdown, effluence of lamellar bodies and thickening of the respiratory membrane. Bleomycin exposure induced expression of MDA, IKKα, phosphorylated IKKα (p-IKKα), NF-κB P65, TNF-α and IL-1β, and reduced I-κB expression in mice lung tissue or in BALF. These changes were reversed by high-dose corilagin (100 mg/kg i.p) more dramatically than by low dose (10 mg/kg i.p). Last, corilagin inhibits TGF-β1 production and α-SMA expression in lung tissue samples. Taken together, these findings confirmed that corilagin attenuates bleomycin-induced epithelial injury and fibrosis via inactivation of oxidative stress, proinflammatory cytokine release and NF-κB and TGF-β1 signaling. Corilagin may serve as a promising therapeutic agent for pulmonary fibrosis.

• Authors: Zheng Wang, Qiong-Ya Guo, Xiao-Ju Zhang, Xiao Li, Wen-Ting Li, Xi-Tao Ma, Li-Jun Ma
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 6 Pg. 9762-79 (May 30 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 24886817 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Glucosides
• Hydrolyzable Tannins
• NF-kappa B
• Transforming Growth Factor beta1
• Tumor Necrosis Factor-alpha
• Bleomycin
• corilagin

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Bleomycin
• Cytokines (analysis, immunology)
• Glucosides (therapeutic use)
• Hydrolyzable Tannins
• Lung (drug effects, immunology, pathology)
• Lung Injury (chemically induced, drug therapy, immunology, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (analysis, immunology)
• Oxidative Stress (drug effects)
• Pulmonary Fibrosis (chemically induced, drug therapy, immunology, pathology)
• Transforming Growth Factor beta1 (analysis, immunology)
• Tumor Necrosis Factor-alpha (analysis, immunology)