Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages.

Abstract

The TNF-α expression-inhibitory effect of lactic acid bacteria (LAB) isolated from kimchi were measured in lipopolysaccharide (LPS)-stimulated peritoneal macrophages. Among the LAB evaluated, Lactobacillus plantarum CLP-0611 inhibited the IL-1β and IL-6 expression, as well as the NF-κB and AP1 activation in LPS-stimulated peritoneal macrophages. Therefore, we investigated its inhibitory effect on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. TNBS significantly induced colon shortening, as well as myeloperoxidase activity and macroscopic score. Oral administration of CLP-0611 significantly reduced TNBS-induced body weight loss, colon shortening, myeloperoxidase activity, IRAK-1 phosphorylation, NF-κB and MAP kinase (p38, ERK, JNK) activation, and iNOS and COX-2 expression. CLP-0611 also inhibited TNBS-induced expression of TNF-α, IL-1β, and IL-6. However, IL-10 expression was induced. CLP-0611 also induced the production of M2 macrophage markers (IL-10, arginase I and CD206). Based on these findings, CLP-0611 inhibits TLR-4-linked NF-κB and MAPK signaling pathways and polarizes M1 to M2-like macrophages, thus ameliorating colitis.

• Authors: Se-Eun Jang, Myung Joo Han, Se-Young Kim, Dong-Hyun Kim
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 21 Issue 1 Pg. 186-92 (Jul 2014) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 24815859 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Lipopolysaccharides
• NF-kappa B
• Transcription Factor AP-1
• Trinitrobenzenesulfonic Acid
• Peroxidase
• Interleukin-1 Receptor-Associated Kinases

Topics

• Administration, Oral
• Animals
• Cell Differentiation
• Cells, Cultured
• Colitis (chemically induced, immunology, microbiology, therapy)
• Colon (pathology)
• Cytokines (genetics, metabolism)
• Interleukin-1 Receptor-Associated Kinases (metabolism)
• Lactobacillus plantarum (immunology)
• Lipopolysaccharides (immunology)
• MAP Kinase Signaling System (immunology)
• Macrophages, Peritoneal (immunology, microbiology)
• Male
• Mice
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Probiotics (administration & dosage)
• Transcription Factor AP-1 (metabolism)
• Trinitrobenzenesulfonic Acid (administration & dosage)