Trichokonins from Trichoderma pseudokoningii SMF2 induce resistance against Gram-negative Pectobacterium carotovorum subsp. carotovorum in Chinese cabbage.

Abstract

Peptaibols, mainly produced by Trichoderma, play a pivotal role in controlling plant disease caused by fungi, virus, and Gram-positive bacteria. In the current study, we evaluated the control effect of Trichokonins, antimicrobial peptaibols from Trichoderma pseudokoningii SMF2, on soft rot disease of Chinese cabbage caused by a Gram-negative bacterium Pectobacterium carotovorum subsp. carotovorum and analyzed the mechanism involved. Trichokonins treatment (0.3 mg L(-1) ) enhanced the resistance of Chinese cabbage against Pcc infection. However, Trichokonins could hardly inhibit the growth of Pcc in vitro, even at high concentration (500 mg L(-1) ). Therefore, the direct effect of Trichokonins on Pcc may not the main reason why Trichokonins could control soft rot of Chinese cabbage. Trichokonin treatment led to an obvious increase in the production of reactive oxygen species hydrogen peroxide and superoxide radical, a significant enhance of the activities of pathogenesis-related enzymes catalase, polyphenoloxidase and peroxidase, and upregulation of the expression of salicylic acid - responsive pathogenesis-related protein gene acidic PR-1a in Chinese cabbage. These results indicate that Trichokonins induce resistance in Chinese cabbage against Pcc infection through the activation of salicylic acid signaling pathway, which imply the potential of Trichoderma and peptaibols in controlling plant disease caused by Gram-negative bacteria.

• Authors: Hai-Yun Li, Yan Luo, Xiu-Sheng Zhang, Wei-Ling Shi, Zhi-Ting Gong, Mei Shi, Lei-Lei Chen, Xiu-Lan Chen, Yu-Zhong Zhang, Xiao-Yan Song
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 354 Issue 1 Pg. 75-82 (May 2014) ISSN: 1574-6968 [Electronic] England
• PMID: 24655217 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

Chemical References

• Alamethicin
• Salicylic Acid

Topics

• Alamethicin (metabolism, pharmacology)
• Brassica (drug effects, genetics, immunology, microbiology)
• Molecular Sequence Data
• Pectobacterium carotovorum (drug effects, growth & development, physiology)
• Plant Diseases (genetics, immunology, microbiology)
• Salicylic Acid (immunology)
• Trichoderma (metabolism)