Antibody produced against isolated Rh(D) polypeptide reacts with other Rh-related antigens.

Abstract

Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobinding and Western blot immunostaining assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as well as the Rh antigen-containing components of sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated membrane proteins from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde), and -D-/-D- cells. It does not react with any membrane protein from Rh-null regulator type cells, thus indicating a specificity for Rh-related proteins. We have also been able to demonstrate that polyclonal and monoclonal anti-D preparations that do not immunostain isolated Rh(D) polypeptide will react with it in our immunobinding assay.

• Authors: K Suyama, J Goldstein
• Journal: Blood (Blood) Vol. 72 Issue 5 Pg. 1622-6 (Nov 1988) ISSN: 0006-4971 [Print] United States
• PMID: 2460158 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antigens
• Epitopes
• Membrane Proteins
• Rh-Hr Blood-Group System
• Rho(D) antigen

Topics

• Antibody Specificity
• Antigens (immunology)
• Blotting, Western
• Cell Membrane (immunology)
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay
• Epitopes
• Humans
• Membrane Proteins (immunology)
• Molecular Weight
• Rh-Hr Blood-Group System (immunology)