Abstract | BACKGROUND: The low efficacy of cancer therapy for the treatment of patients with advanced disease makes the development of new anticancer agents necessary. Because natural products are a significant source of anticancer drugs, it is important to explore cytotoxic activity of novel compounds from natural origin. PURPOSE: MATERIAL AND METHODS: The cytotoxic ability of Alnus glutinosa leaves ethyl acetate extract was studied over HeLa and PC-3 cell lines, with the MTT colorimetric assay. Hirsutanone was isolated from this extract using chromatographic methods, and its structure elucidated by spectroscopic analysis. HT-29 cell viability after hirsutanone treatment was determined using SRB assay. In order to understand hirsutanone way of action, cytotoxicity was evaluated adding the diarylheptanoid and antioxidants. DNA topoisomerase II ( topo II) poison activity, was also evaluated using purified topo II and a supercoiled form of DNA that bears specific topo II recognition and binding region; topo II poisons stabilize normally transient DNA- topo II cleavage complexes, and lead an increased yield of linear form as a consequence of a lack of double-strand breaks rejoining. RESULTS: CONCLUSION: Our data suggest that, like curcumin, an induction of oxidative stress and topo II-mediated DNA damage may play a role in hirsutanone-induced cancer cell death. Since both compounds share similar structure and cytotoxic profile, and curcumin is in clinical trials for the treatment of cancer, our results warrant further studies to evaluate the anticancer potential of hirsutanone.
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Authors | A J León-Gonzalez, N Acero, D Muñoz-Mingarro, M López-Lázaro, C Martín-Cordero |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 21
Issue 6
Pg. 866-70
(May 15 2014)
ISSN: 1618-095X [Electronic] Germany |
PMID | 24581747
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier GmbH. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Diarylheptanoids
- Plant Extracts
- Reactive Oxygen Species
- DNA
- DNA Topoisomerases, Type II
- hirsutanone
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Topics |
- Adenocarcinoma
(drug therapy)
- Alnus
(chemistry)
- Antineoplastic Agents, Phytogenic
(isolation & purification, pharmacology, therapeutic use)
- Cell Death
- Colonic Neoplasms
(drug therapy, metabolism)
- DNA
(drug effects)
- DNA Topoisomerases, Type II
(metabolism)
- Diarylheptanoids
(isolation & purification, pharmacology, therapeutic use)
- HT29 Cells
- Humans
- Phytotherapy
- Plant Extracts
(chemistry, pharmacology, therapeutic use)
- Plant Leaves
- Reactive Oxygen Species
(metabolism)
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