Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells.

Abstract

Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.

• Authors: Flávio de Oliveira Silva, Priscila das Neves Santos, Evellyne de Oliveira Figueirôa, Cristiane Moutinho Lagos de Melo, Juliana Kelle de Andrade Lemoine Neves, Francisco Vassiliepe Sousa Arruda, João Batista Cajazeiras, Kyria Santiago do Nascimento, Edson Holanda Teixeira, Benildo Sousa Cavada, Ana Lúcia Figueiredo Porto, Valéria Rêgo Alves Pereira
• Journal: Research in veterinary science (Res Vet Sci) Vol. 96 Issue 2 Pg. 276-82 (Apr 2014) ISSN: 1532-2661 [Electronic] England
• PMID: 24565003 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• Plant Lectins
• lectin, Canavalia
• Nitric Oxide

Topics

• Animals
• Apoptosis (drug effects)
• Canavalia (immunology)
• Cell Line, Tumor
• Cell Movement (immunology)
• Cell Survival (immunology)
• Cytokines (analysis)
• Flow Cytometry
• Melanoma (drug therapy, immunology)
• Mice
• Nitric Oxide (analysis)
• Plant Lectins (pharmacology, therapeutic use)
• Statistics, Nonparametric