Abstract |
The effects of protein kinase C stimulation on free cytosolic Ca2+ [( Ca2+]i) were studied in Fura 2-loaded UMR-106 cells. Stimulation of the protein kinase C with the tumor-promoting phorbol esters 12-O-tetradecanoylphorbol 13-acetate (TPA) and phorbol 12,13-diacetate or 1-oleoyl-2-acetylglycerol was followed by an increase in [Ca2+]i. The protein kinase C-induced increase in [Ca2+]i has a lag period, the duration of which was dependent on the stimulant and medium Ca2+ concentrations. With 2 microM TPA, the rise in [Ca2+]i peaked within 1.5 min, after which [Ca2+]i returned partially toward base line. The increase in [Ca2+]i was absolutely dependent on the presence of medium Ca2+ and was inhibited by the Ca2+ channel blockers nicardipine and verapamil. Cell stimulation also results in Ca2+ release from intracellular pool(s) which appears to be mediated by a Ca2+-dependent Ca2+ release mechanism. The reduction in [Ca2+]i was due to channel inactivation. Pretreatment of the cells with 1 nM TPA, 2 units/ml parathyroid hormone (PTH), or 15 microM forskolin blocked the effect of 2 microM TPA on [Ca2+]i. TPA and PTH were more potent inhibitors than was forskolin. The properties of this channel are compared to the cAMP-independent PTH-stimulated Ca2+ channel present in these cells.
|
Authors | D T Yamaguchi, C R Kleeman, S Muallem |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 262
Issue 31
Pg. 14967-73
(Nov 05 1987)
ISSN: 0021-9258 [Print] United States |
PMID | 2444593
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- Calcium Channel Blockers
- Ion Channels
- Phorbol Esters
- Colforsin
- Protein Kinase C
- Tetradecanoylphorbol Acetate
- Calcium
|
Topics |
- Calcium
(metabolism)
- Calcium Channel Blockers
(pharmacology)
- Cell Line
- Colforsin
(pharmacology)
- Ion Channels
(drug effects, metabolism)
- Kinetics
- Osteoblasts
(metabolism)
- Osteosarcoma
- Phorbol Esters
(pharmacology)
- Protein Kinase C
(pharmacology)
- Tetradecanoylphorbol Acetate
(pharmacology)
|