Cyanidin-3-O-sambubioside from Acanthopanax sessiliflorus fruit inhibits metastasis by downregulating MMP-9 in breast cancer cells MDA-MB-231.

Abstract

Anthocyanins play an important role in physiological functions related to human health. This study aimed to investigate the inhibitory effect of cyanidin-3-O-sambubioside isolated from Acanthopanax sessiliflorus fruit on cancer cell metastasis. The inhibitory effect of cyanidin-3-O-sambubioside on angiogenesis was assessed by chorioallantoic membrane and tube formation assays. Cyanidin-3-O-sambubioside interrupted the formation of neovasculature and tube-like structures in a dose-dependent manner. It inhibited the wound healing migration and invasion of MDA-MB-231 cells through reconstituted extracellular matrix (Matrigel). Gelatin zymography revealed that cyanidin-3-O-sambubioside significantly decreased the secretion of matrix metalloproteinase-9 and inhibited matrix metalloproteinase-9 expression. In additional, cyanidin-3-O-sambubioside exerted an inhibitory effect on Akt phosphorylation. These results suggest that cyanidin-3-O-sambubioside inhibits metastasis processes, such as angiogenesis and invasion, in breast cancer cells through regulation of matrix metalloproteinase-9 activity.

• Authors: Sang-Jin Lee, Sungyoul Hong, Sang-Ho Yoo, Gye-Won Kim
• Journal: Planta medica (Planta Med) Vol. 79 Issue 17 Pg. 1636-40 (Nov 2013) ISSN: 1439-0221 [Electronic] Germany
• PMID: 24214832 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Angiogenesis Inhibitors
• Anthocyanins
• Antineoplastic Agents, Phytogenic
• Disaccharides
• NF-kappa B
• Plant Extracts
• cyanidin 3-sambubioside
• Proto-Oncogene Proteins c-akt
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Angiogenesis Inhibitors (pharmacology, therapeutic use)
• Animals
• Anthocyanins (pharmacology, therapeutic use)
• Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
• Breast Neoplasms (drug therapy, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Chick Embryo
• Chorioallantoic Membrane
• Disaccharides
• Dose-Response Relationship, Drug
• Down-Regulation
• Eleutherococcus (chemistry)
• Extracellular Matrix
• Female
• Fruit (chemistry)
• Humans
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• NF-kappa B (metabolism)
• Neovascularization, Pathologic
• Phosphorylation
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Proto-Oncogene Proteins c-akt (metabolism)