Abstract |
Antimitotic agents are frequently used to treat solid tumors and hematologic malignancies. However, one major limitation of antimitotic approaches is mitotic slippage, which is driven by slow degradation of cyclin B during a mitotic block. The extent to which cyclin B levels decline is proposed to be governed by an equilibrium between cyclin B synthesis and degradation. It was recently shown that the 3' untranslated region (UTR) of the murine cyclin B mRNA contributes to the synthesis of cyclin B during mitosis in murine cells. Using a novel live-cell imaging-based technique allowing us to study synthesis and degradation of cyclin B simultaneously at the single cell level, we tested here the role of the human cyclin B 3'UTR in regulating cyclin B synthesis during mitosis in human cells. We observed that the cyclin B 3'UTR was not sufficient to enhance cyclin B synthesis in human U2Os, HeLa or hTERT RPE-1 cells. A better understanding of how the equilibrium of cyclin B is regulated in mitosis may contribute to the development of improved therapeutic approaches to prevent mitotic slippage in cancer cells treated with antimitotic agents.
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Authors | Dominik Schnerch, Marie Follo, Julia Felthaus, Monika Engelhardt, Ralph Wäsch |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 9
Pg. e74379
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24058555
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3' Untranslated Regions
- Cyclin B
- RNA, Messenger
- Paclitaxel
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Topics |
- 3' Untranslated Regions
(genetics)
- Animals
- Cell Line
- Chromosomes, Human
(metabolism)
- Cyclin B
(biosynthesis, genetics)
- Genes, Reporter
- Humans
- Kinetics
- Mice
- Microtubules
(drug effects, metabolism)
- Mitosis
(drug effects, genetics)
- Paclitaxel
(pharmacology)
- Proteolysis
(drug effects)
- RNA, Messenger
(genetics, metabolism)
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